Tolerance Data 2009.2 Keygen 42 REPACK
Tolerance Data 2009.2 Keygen 42
tolerance to ct26.wt murine colon carcinoma cells, which express the model antigen ovalbumin (ova), was assessed in mice injected with ct26.ova cells (2.5×106) on day 0 and day 7. ct26.ova cells express the ova gene, which is under control of the k5 promoter. antigen-specific t cells were observed only in the ct26.ova tumor-bearing mice (2.8% of splenocytes) but not in naive mice (0.07% of splenocytes; figure 3 ). the ova-specific t cells in ct26.ova tumors were cd3+, cd4+, cd8-, cd62l-, and cd127-. tregs were identified using foxp3 as a marker. the frequency of tregs was increased in ct26.ova tumor bearing mice compared to naive mice (5.2±0.8 vs 0.8±0.2% of splenocytes; figure 3 ). foxp3+ cells were exclusively localized within the ct26.ova tumor (figure 4 ).
in this study we evaluated a recently developed high throughput method for the quantification of 18s rrna in human serum. first, we investigated whether an adequate baseline level of rrna can be found in human serum and compared this to the corresponding value in cultured human heparg cells. second, we compared the performance of the quant-it picogreen dsdna assay to a conventional spectrophotometric assay for total dna. third, we measured serum rrna levels in patients with type 2 diabetes and compared them with healthy control subjects. fourth, we determined the levels of serum rrna in patients with preeclampsia and gestational diabetes mellitus. fifth, we compared the levels of serum rrna in patients with gestational diabetes who had normal glucose tolerance (ngt) or who developed preeclampsia at follow-up. finally, we measured rrna levels in serum samples from pregnant women with normal glucose tolerance and compared them with those with gestational diabetes mellitus at follow-up. serum samples from the different study groups were all drawn between the 24th and 28th week of pregnancy.
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