Bunifu UI WinForms 1.7.0.0
Bunifu UI WinForms 1.7.0.0
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3.3. Enzymatic Assay of NDs {#sec3.3}
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Prior to enzymatic assays of NDs, a spectrophotometric assay was performed to ensure that the NDs did not interfere with the proposed reaction catalyzed by ASE. In this assay, 10 mL of 6 *μ*M thioflavin T in 50 mM sodium phosphate buffer (pH 7.0) was mixed with 1 mg of NDs in a 5 mL cuvette. The mixture was incubated in a spectrophotometer at 25°C in an RT. Fluorescence was monitored at thioflavin T excitation and emission wavelengths of 442 and 482 nm, respectively.
3.4. Enzymatic Assay of NDs {#sec3.4}
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In each assay, ASE in 1 × 100 *μ*L of 50 mM sodium phosphate buffer (pH 7.0) containing 6 *μ*M thioflavin T
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