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Oct 24, 2011n/a2:00AM – Closing time for open account, please wait. BIOHAZARK GAME SET +CD Key!FREE!!Measurement of subendocardial myocardial blood flow and left ventricular function with myocardial contrast echocardiography in pigs. The feasibility of monitoring subendocardial myocardial blood flow and left ventricular function at rest and during stress was assessed in seven chronically instrumented 12-14-week-old (average 130 kg) domestic pigs. Using myocardial contrast echocardiography, which is based on ultrasound backscatter, studies were made with three consecutive power injections at 8, 12, and 16 microliters/kg during spontaneous breathing. The backscatter signals were recorded simultaneously at the midwall on the short-axis views of the left ventricle. The myocardial blood flow values were calculated as mean transit time corrected to tracer concentration and divided by myocardial volume. Left ventricular function was assessed during rest and stress (tertiary transmural stimulation) of the left ventricle, with and without dobutamine (5 micrograms.kg-1.min-1) infusion, to mimic a 5% and 10% increase in contractile force from rest. During tracer injection, T(1/2) values measured by backscatter were as follows: a) midwall: 31 +/- 4% and 2.2 +/- 1.6 min with 8, 12, and 16 microliters/kg; b) epicardium: 63 +/- 23% and 2.2 +/- 0.7 min with 8, 12, and 16 microliters/kg; c) endocardium: 81 +/- 33% and 2.5 +/- 0.8 min with 8, 12, and 16 microliters/kg. Only the first T(1/2) values were significantly different from the other T(1/2) values (P < 0.05). The left ventricular end-systolic volume and ejection fraction values decreased from rest to supine position (rest: 188 +/- 10, 85 +/- 2 and 77 +/- 3 ml; supine: 143 +/- 7, 66 +/- 3 and 59 +/- 3 ml, respectively) and increased significantly from supine to stress position (supine: 143 +/- 7, 82 +/- 3, 74 +/- 3 ml; stress: 208 +/- 18, 86 +/- 2, 77 +/- 2 ml

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